A REVIEW OF 2-FDCK HCL

The lab says fixation will cause the dyes to leak within the cells. I believe the idea is fixation may destruction mobile membranes. I did find a paper which describes fixation applying methanol or acetic acid, right after staining using a DCFDA derivative. DCFDA would be the reagent that stains ROS in the package ab113851, and I think that it's th